This module describes the basic features and properties of proteins and their relation to protein function. It provides hands on experience in the production, isolation, purification and characterisation of proteins with biotechnological potential. The relationship of protein structure to function is examined in light of the mechanisms of enzyme catalysis, signal transduction and other important biological processes. The techniques used in protein purification are described and the laboratory classes provide experience in protein purification and analysis. In addition, you will obtain theoretical and practical experience of obtaining and analysing proteomic data.
Aims
The module aims to provide students with the necessary knowledge, skills and hand-on experience in protein production, purification and analysis.
Learning Outcomes
In order to pass this module the student will need to be able to:
1. Explain the factors that determine protein structure and function;
2. Be able to discuss the different levels of protein structural organization;
3. Explain how proteins are synthesized in the cell and how their function is regulated by alternative splicing and post-translational modification;
4. Discuss protein targeting in prokaryotic and eukaryotic cells;
5. Describe the range of techniques used in protein analysis;
6. Discuss the methods of production of recombinant proteins;
7. Describe how different types of chromatography can be combined to achieve better protein purification;
8. Describe gel electrophoresis and its application for protein analysis;
9. Understand the principles of proteomics and its applications;
10. Demonstrate practical competence in key protein purification and analysis techniques;
11. Develop a range of key skills including information acquisition from web-based and library sources, self-directed learning, numeracy, writing and presentation of scientific reports.
Aims
The module aims to provide students with the necessary knowledge, skills and hand-on experience in protein production, purification and analysis.
Learning Outcomes
In order to pass this module the student will need to be able to:
1. Explain the factors that determine protein structure and function;
2. Be able to discuss the different levels of protein structural organization;
3. Explain how proteins are synthesized in the cell and how their function is regulated by alternative splicing and post-translational modification;
4. Discuss protein targeting in prokaryotic and eukaryotic cells;
5. Describe the range of techniques used in protein analysis;
6. Discuss the methods of production of recombinant proteins;
7. Describe how different types of chromatography can be combined to achieve better protein purification;
8. Describe gel electrophoresis and its application for protein analysis;
9. Understand the principles of proteomics and its applications;
10. Demonstrate practical competence in key protein purification and analysis techniques;
11. Develop a range of key skills including information acquisition from web-based and library sources, self-directed learning, numeracy, writing and presentation of scientific reports.
- Module Supervisor: Metodi Metodiev